Research Fellow - Lab Medicine and Pathology
The candidate will be working under the mentorship of Dr. Maia and Dr. Harrington in a joint project to define transcriptional dependencies associated with serous (p53 Aberrant) endometrial cancer.
Responsibilities:
- Cultivate mammalian cell cultures, primarily intestinal organoid/tumoroid models
- Derive and establish normal and cancer organoid lines frompatient samples
- Design and conduct drug screenings and evaluate the efficacy of novel therapeutics in preclinical organoid models.
- Use reporter expressing organoid models to assess cell state dynamics, in response to various compounds and drug candidates, using flow cytometry analysis.
- Conduct a wide range of molecular biology techniques, such as DNA/RNA extraction, PCR, qPCR, cloning, western blotting and chromatin immunoprecipitation (ChiP).
- Interpret, present, and document experiments based on generated data to investigators
- Maintain and report accurate records of data
- Experience with genomics techniques such as ATAC-seq, RNA-seq, ChiP-seq, CUT&RUN is preferred.
- Experience within vivo preclinical models of oncology is also preferred.
Qualifications
- PhD in a relevant field (e.g., cancer biology, molecular biology, genetics, or related disciplines).
- Proficiency in a wide range of molecular biology techniques, including DNA/RNA extraction, PCR, qPCR, cloning, western blotting, chromatin immunoprecipitation (ChiP) and CUT&RUN.
- Proficiency in mammalian cell cultures—particularly organoids.
- Proficiency in bioinformatic analysis.
- Excellent organizational skills and attention to detail.
- Ability to work independently as well as collaboratively in a team-oriented environment.
- Effective communication and interpersonal skills.
